An Integrated Microfluidic Device for High-throughput Electrophysiological Analysis of C. Elegans
نویسندگان
چکیده
The traditional method for recording electrophysiological signals from the C. elegans pharynx is called an electropharyngeogram (EPG). It provides an experimental platform for defining gene function, the impact of genetic mutations, and the effects of drugs on the nematode neural network. To date, the EPG has been a low-throughput assay. This paper describes a new microfluidic device that can sequentially trap worms and capture EPG activity combined with the capability to apply drug solutions. With the integration of micro-valves and embedded electrodes, we are developing a new tool for consistent, high-quality and high-throughput neurogenetic and neuropharmacological analysis of nematodes.
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